RESUMO: Lucidotini Lacordaire 1857 is a highly diverse and remarkably widespread lineage of fireflies, with approximately 850 species distributed across 34 recognized genera and 4 currently accepted subtribes: Dadophorina Olivier 1907, Lucidotina Lacordaire 1857, Phosphaenina McDermott 1964, and Photinina LeConte 1881. The taxonomy of Lucidotini has long been hindered and complicated by outdated or imprecise diagnoses, and their phylogenetic affinities remain poorly understood. Traditionally, Phosphaenina includes the genera Phosphaenus Laporte 1833 and Phosphaenopterus Schaufuss 1870, but recent phylogenetic analyses have suggested close affinities to other lineages, which challenged the monophyly of Lucidotini subtribes. In the present study, we reassess the composition, limits, and defining characters of Phosphaenina based on a phylogenetic analysis of 30 taxa and 94 morphological characters under Maximum Parsimony and Bayesian Inference. Our results support a comprehensive redefinition of Phosphaenina s.n. to include: Dinophallus gen. n., Dociluta gen. n., Lucidina Gorham 1883, Luciuranus Silveira et al. 2016, Phosphaenopterus, Phosphaenus, and Streptophallus gen. n., and Lucidota partim (Lucidota atra [Olivier 1790], Lucidota punctata [LeConte 1852], and Dociluta pygmea comb. n.). We also describe 3 new genera and 4 new species and provide new diagnostic traits and an updated key to the genera of Phosphaenina s.n. Among the traits supporting Phosphaenina s.n. is a joint anchoring the phallic dorsal plate to the parameres, described herein. These findings underscore the value of comprehensive morphological studies in clarifying the taxonomy and evolutionary relationships within Lucidotini.